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Histone 3 antibody (H3K9me2, H3K9me3)

The Mouse Monoclonal anti-Histone 3 antibody has been validated for WB, ELISA, ChIP, DB and IHC (fro). It is suitable to detect Histone 3 in samples from Human and Cow.
Catalog No. ABIN1169690

Quick Overview for Histone 3 antibody (H3K9me2, H3K9me3) (ABIN1169690)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Cow

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

6F12-H4
  • Binding Specificity

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    H3K9me2, H3K9me3

    Specificity

    Specific for K9 tri-methylated histone H3, weak cross-reactivity with K9 di- and even less monomethylated histone H3 but not unmethylated K9

    Cross-Reactivity (Details)

    Reacts with Human and cow. predicted to detect many other species due to sequence identity such as Mouse, Rat, Rabbit, Hamster, Pig, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Non Human Primates

    Purification

    Protein G

    Immunogen

    Synthetic peptide of Human Histone H3, tri methylated at K9

    Isotype

    IgG1
  • Application Notes

    Optimal antibody dilution should be determined by titration, however as a guideline try up to 1:1000 for western blot. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 peptide - tri methyl K9 

    Comment

    Myeloma, fusion partners: X63-Ag8.653

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Background

    Synonyms:
    Histone H3.1 antibody,FLJ92264 antibody,H3 histone antibody,H3 histone family, member A antibody,H3/a antibody,H3/b antibody,H3/c antibody,H3/d antibody,h3/f antibody,H3/h antibody,H3/i antibody,H3/j antibody,H3/k antibody,H3/l antibody,H31_HUMAN antibody,H3F1K antibody,H3F3 antibody,H3FA antibody,H3FB antibody,H3FC antibody,H3FD antibody,H3FF antibody,H3FH antibody,H3FI antibody,H3FJ antibody,H3FK antibody,H3FL antibody,HIST1H3A antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody

    Gene ID

    100364501, 100365669, 291159

    OMIM

    601128

    UniProt

    P68432, P02299
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