IGFBP3 antibody (FITC)

Catalog No. ABIN1172104

This Rabbit Polyclonal antibody specifically detects IGFBP3 in WB, IHC, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for IGFBP3 antibody (FITC) (ABIN1172104)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IGFBP3 Antibody (FITC)

Purpose: FITC-Linked Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Specificity: The antibody is a rabbit polyclonal antibody raised against IGFBP3. It has been selected for its ability to recognize IGFBP3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: The antibody is a rabbit polyclonal antibody raised against IGFBP3 conjugated to fitc.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: Insulin Like Growth Factor Binding Protein 3

Background: BP53, IBP3, Growth Hormone-Dependent Binding Protein, Acid Stable Subunit Of The 140 K IGF Complex

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding