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aHSP antibody (Biotin)

This anti-aHSP antibody is a Rabbit Polyclonal antibody detecting aHSP in WB, IHC and ICC. Suitable for Human.
Catalog No. ABIN1172862

Quick Overview for aHSP antibody (Biotin) (ABIN1172862)

Target

See all aHSP Antibodies
aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This aHSP antibody is conjugated to Biotin

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Purpose

    Biotin-Linked Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against aHSP. It has been selected for its ability to recognize aHSP in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    PAA412Hu01Polyclonal Antibody to AlphaHemoglobin Stabilizing Protein (aHSP)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze/thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

    Alternative Name

    Alpha-Hemoglobin Stabilizing Protein

    Background

    A-HSP, ERAF, EDRF, Erythroid Associated Factor, Erythroid differentiation-related factor
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