KRT16 antibody (AA 117-419) (Biotin)

Catalog No. ABIN1172995

This Rabbit Polyclonal antibody specifically detects KRT16 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for KRT16 antibody (AA 117-419) (Biotin) (ABIN1172995)

Target: KRT16 (Keratin 16 (KRT16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT16 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-KRT16 Antibody (Biotin)

Binding Specificity: AA 117-419

Purpose: Biotin-Linked Polyclonal Antibody to Cytokeratin 16 (CK16)

Sequence: MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDD DDKAMADIGSEF-EKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY QILLDVKTRL EQEIATYRR

Specificity: The antibody is a rabbit polyclonal antibody raised against CK16. It has been selected for its ability to recognize CK16 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant KRT16 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against KRT16 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: Cytokeratin 16

Background: KRT16, NEPPK, Keratin 16, Focal Non-Epidermolytic Palmoplantar Keratoderma, Keratin, type I cytoskeletal 16