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Dopamine antibody (FITC)

This Rabbit Polyclonal antibody specifically detects Dopamine in WB, ICC, IHC and IF. It exhibits reactivity toward Various Species.
Catalog No. ABIN1173790

Quick Overview for Dopamine antibody (FITC) (ABIN1173790)

Target

See all Dopamine (DA) Antibodies
Dopamine (DA)

Reactivity

  • 5
  • 2
Various Species

Host

  • 12
  • 5
Rabbit

Clonality

  • 12
  • 5
Polyclonal

Conjugate

  • 12
  • 3
  • 1
  • 1
This Dopamine antibody is conjugated to FITC

Application

  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Purpose

    FITC-Linked Polyclonal Antibody to Dopamine (DA)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against DA. It has been selected for its ability to recognize DA in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Various Species

    Characteristics

    Molecular Mass: 153.18 g/mol

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    PAA851Ge01Polyclonal Antibody to Dopamine (DA)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze/thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Dopamine (DA)

    Alternative Name

    Dopamine

    Target Type

    Chemical

    Background

    2-(3,4-Dihydroxyphenyl)ethylamine, 3,4-Dihydroxyphenethylamine, 3-Hydroxytyramine, Intropin, Revivan, Oxytyramine, Dopamine Hydrochloride
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