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Hemoglobin antibody

This anti-Hemoglobin antibody is a Rabbit Polyclonal antibody detecting Hemoglobin in WB, IHC, ICC and IP. Suitable for Duck.
Catalog No. ABIN1174581

Quick Overview for Hemoglobin antibody (ABIN1174581)

Target

See all Hemoglobin Antibodies
Hemoglobin

Reactivity

  • 78
  • 24
  • 17
  • 15
  • 13
  • 12
  • 6
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
Duck

Host

  • 110
  • 37
  • 30
  • 9
  • 4
  • 4
  • 1
  • 1
Rabbit

Clonality

  • 152
  • 37
  • 1
Polyclonal

Conjugate

  • 79
  • 48
  • 29
  • 22
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This Hemoglobin antibody is un-conjugated

Application

  • 125
  • 105
  • 104
  • 45
  • 20
  • 12
  • 11
  • 10
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • Purpose

    Polyclonal Antibody to Hemoglobin (HB)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against HB. It has been selected for its ability to recognize HB in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Native Protein Hemoglobin is the iron-containing oxygen-transport metalloprotein in the red blood cells of all vertebrates as well as the tissues of some invertebrates. Hemoglobin in the blood carries oxygen from the respiratory organs (lungs or gills) to the rest of the body where it releases the oxygen to burn nutrients to provide energy to power the functions of the organism, and collects the resultant carbon dioxide to bring it back to the respiratory organs to be dispensed from the organism
    The antibody is a rabbit polyclonal antibody raised against HB.

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze/thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Hemoglobin

    Background

    Hgb, Haemoglobin, Heterotetramer(αβ)2
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