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CUBN antibody (Biotin)

This anti-CUBN antibody is a Rabbit Polyclonal antibody detecting CUBN in WB, IHC and ICC. Suitable for Rat.
Catalog No. ABIN1175679

Quick Overview for CUBN antibody (Biotin) (ABIN1175679)

Target

See all CUBN Antibodies
CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Reactivity

  • 46
  • 14
  • 12
Rat

Host

  • 68
  • 2
Rabbit

Clonality

  • 58
  • 12
Polyclonal

Conjugate

  • 28
  • 11
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CUBN antibody is conjugated to Biotin

Application

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  • 24
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  • 15
  • 6
  • 4
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  • 3
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Purpose

    Biotin-Linked Polyclonal Antibody to Cubilin (CUBN)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against CUBN. It has been selected for its ability to recognize CUBN in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    The antibody is a rabbit polyclonal antibody raised against CUBN conjugated to biotin.

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze/thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

    Alternative Name

    Cubilin

    Background

    IFCR, MGA1, gp280, Intrinsic Factor Cobalamin Receptor, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-vitamin B12 receptor

    Pathways

    Metabolism of Steroid Hormones and Vitamin D, Lipid Metabolism
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