ESM1 antibody (AA 20-184)

Catalog No. ABIN1175696

The Rabbit Polyclonal anti-ESM1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ESM1 in samples from Human. There is 1 publication available.

Quick Overview for ESM1 antibody (AA 20-184) (ABIN1175696)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ESM1 Antibody

Binding Specificity: AA 20-184

Purpose: Polyclonal Antibody to Endothelial Cell Specific Molecule 1 (ESM1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ESM1. It has been selected for its ability to recognize ESM1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Endothelial Cell Specific Molecule 1 (ESM1) corresdonding to Trp20~Arg184 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ESM1 antibody (ABIN1175696)

Sun, Sun, Sun, Yang: "Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity." in: Molecular medicine reports, Vol. 19, Issue 4, pp. 3247-3254, (2019) (PubMed).

Target Details for ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: ESM1

Background: Endocan

Pathways: Growth Factor Binding