CCL2 antibody

Catalog No. ABIN1176870

This anti-CCL2 antibody is a Armenian Hamster Monoclonal antibody detecting CCL2 in ELISA, Neut and ICS. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CCL2 antibody (ABIN1176870)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Clone: 2H5

Product Details anti-CCL2 Antibody

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse MCP-1. Neutralization activity is measured in a calcium flux assay using 100-200 ng/mL recombinant mouse MCP-1 (Cat. No. 554590) to stimulate THP-1 indicator cells.
50% Neutralization (ND50) at 2 - 30 myg/mL
90% Neutralization at 40 - 300 myg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Heparin-purified CHO-expressed mouse MCP-1

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: The purified format of the 2H5 antibody (Cat. No. 551217) is useful as a capture antibody in a sandwich ELISA for measuring mouse MCP-1 protein levels when paired with biotin conjugated clone 4E2/MCP (Cat. No, 554444) as a detection reagent and recombinant mouse MCP-1 (Cat. No. 554590) as the standard. For testing mouse MCP-1 in complex biological fluids, such as serum or plasma, investigators are encouraged to consider using the BD OptEIA™ Mouse MCP-1 Set (Cat. No. 555260).
Flow Cytometry: The 2H5 antibody can be useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate MCP-1 producing cells within mixed cell populations. The PE-conjugated 2H5 antibody (Cat. No. 554443) is especially suitable for these experiments.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CCL2 antibody (ABIN1176870)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Luo, Laning, Hayashi, Hancock, Rollins, Dorf: "Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3708-16, (1994) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP-1

Background: The 2H5 antibody reacts with mouse and rat monocyte chemoattractant protein (MCP-1), formerly termed JE. This antibody also recognizes human MCP-1, but shows no reactivity with the closely related mouse beta chemokines, TCA3 and MIP-1beta. The immunogen used to generate the 2H5 hybridoma was heparin-purified CHO-expressed mouse MCP-1. This is a neutralizing antibody.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection