Cyanine Dyes antibody (Biotin)

Catalog No. ABIN117887

The Sheep Polyclonal anti- antibody has been validated for IF, EIA, FACS and IHC (fro). It is suitable to detect in samples from .

Quick Overview for Cyanine Dyes antibody (Biotin) (ABIN117887)

Target: Cyanine Dyes

Host: Sheep

Clonality: Polyclonal

Conjugate: Biotin

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Characteristics: Molar Ratio: 10-20 BAC molecule per sheep IgG molecule.

Purification: Immunoaffinity chromatography

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assay using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: This product has been assayed against 1.0 μg of Cyanine IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative and 10 mg/mL BSA as stabilzer, IgG and Protease free.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: Cyanine Dyes

Target Type: Chemical