Cyanine Dyes antibody (HRP)

Catalog No. ABIN117889

This Sheep Polyclonal antibody specifically detects in IP. It exhibits reactivity toward .

Quick Overview for Cyanine Dyes antibody (HRP) (ABIN117889)

Target: Cyanine Dyes

Host: Sheep

Clonality: Polyclonal

Conjugate: HRP

Application: Immunoprecipitation (IP)

Product Details

Purification: Immunoaffinity chromatography

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Application Details

Application Notes: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring extremely low background levels, absence of F(c) mediatedbinding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 g of Cyanineconjugated Sheep IgG in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,500 to 1: 6,000 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: Cyanine Dyes

Target Type: Chemical