CD8 antibody

Catalog No. ABIN118133

The Rat Monoclonal anti-CD8 antibody has been validated for FACS, IF and IHC (fro). It is suitable to detect CD8 in samples from Mouse.

Quick Overview for CD8 antibody (ABIN118133)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KT15

Product Details anti-CD8 Antibody

Specificity: This antibody recognizes Ly2.1 and Ly2.2 cells, reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.

Purification: Affinity Chromatography on Protein A

Immunogen: T cell clone, C6. Remarks: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/100 - 1/200, use 10 μL of the suggested working dilution to label 10^6cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731