CD161 antibody

Catalog No. ABIN118295

The Mouse Monoclonal anti-CD161 antibody has been validated for FACS, IP and CyTox. It is suitable to detect CD161 in samples from Mouse.

Quick Overview for CD161 antibody (ABIN118295)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Clone: PK136

Product Details anti-CD161 Antibody

Specificity: This antibody (clone PK136) recognises the NK1.1 cell surface antigen. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A

Immunogen: Spleen and bone marrow cells from CE mice. Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: use 10 μL of 5-10 μg/mL diluted antibody to label 1 x 10^6 cells in 100 μL. Immunoprecipitation. Cytotoxic Assays: 1-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161 / KLRB1

Background: NK1.1 is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

Gene ID: 3820

NCBI Accession: NP_002249

UniProt: Q12918