Interferon gamma antibody

Catalog No. ABIN118372

This Rat Monoclonal antibody specifically detects Interferon gamma in FACS and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Interferon gamma antibody (ABIN118372)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: XMG1-2

Product Details anti-Interferon gamma Antibody

Specificity: This antibody recognises IFN gamma. This reagent may be used for the detection of intracellular IFN gamma using flow cytometric techniques.

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/50, Membrane permeablisation is required. ELISA: 1 μg/mL - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 15978

NCBI Accession: NP_032363

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy