CD3 antibody (FITC)

Catalog No. ABIN118478

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Human and Primate.

Quick Overview for CD3 antibody (FITC) (ABIN118478)

Target: CD3

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: UCHT1

Product Details anti-CD3 Antibody (FITC)

Specificity: This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19 kDa. As a pan T cell marker this antibody is present on more than 95 % of circulating human peripheral T cells.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells or 100 μL of lysed whole peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This antibody is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3 is a surface protein of peripheral blood T cells of molecular weight 19 kD. The pan T cell marker is present on more than 95 % of circulating human peripheral T cells.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway