ITGAL antibody

Catalog No. ABIN118558

This Mouse Monoclonal antibody specifically detects ITGAL in FACS, IP, Func and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ITGAL antibody (ABIN118558)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 38

Product Details anti-ITGAL Antibody

Specificity: This antibody recognises the integrin alphaL subunit, a 180 kDa glycoprotein expressed by all human leucocytes. The molecule is involved in cell-cell adhesion processes and binds to ICAM-1, 2 and 3. This antibody neutralises CD11a mediated adhesion.

Purification: Affinity chromatography on Protein G

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 5 μL of the suggested working dilution to label 10^6 cells in 100 μL. Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections. Recommended positive control tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a / ITGAL

Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gene ID: 3683

NCBI Accession: NP_002200

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex