CD33 antibody (APC)

Catalog No. ABIN118633

This Mouse Monoclonal antibody specifically detects CD33 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD33 antibody (APC) (ABIN118633)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: WM53

Product Details anti-CD33 Antibody (APC)

Specificity: This antibody recognises the CD33 cell surface glycoprotein.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for CD33

Target: CD33

Alternative Name: CD33 / SIGLEC3

Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID: 945

NCBI Accession: NP_001763

UniProt: P20138