ITGA6 antibody

Catalog No. ABIN118734

Product Details anti-ITGA6 Antibody

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.

Purification: Affinity Chromatography on Protein G.

Immunogen: a6b4 integrin purified from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Clone: 450-30A

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: CD49f / ITGA6 (ITGA6 Products)

Background: Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6

Gene ID: 3655

NCBI Accession: NP_000201

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex