CCL5 antibody

Catalog No. ABIN118992

This anti-CCL5 antibody is a Mouse Monoclonal antibody detecting CCL5 in WB, IP, Func, EIA, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for CCL5 antibody (ABIN118992)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5B3-D5

Product Details anti-CCL5 Antibody

Specificity: Recognizes Human RANTES chemokine. Other species not tested.

Purification: Affinity Chromatography on Protein A.

Immunogen: E. Coli derived recombinant Human Rantes (Cat#PA123)

Isotype: IgG2b

Application Details

Application Notes: ELISA: A 1/8,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in a direct ELISA using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories). Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. Also reported to work in: Immunoprecipitation, Immunohistochemistry on Frozen sections and Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water, the antibody is stable for six weeks at 2-8 °C.

Expiry Date: 12 months

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES / CCL5

Background: RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5. This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1. CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gene ID: 6352

NCBI Accession: NP_002976

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration