CD8 antibody (FITC)

Catalog No. ABIN119442

This anti-CD8 antibody is a Rat Monoclonal antibody detecting CD8 in FACS. Suitable for Human.

Quick Overview for CD8 antibody (FITC) (ABIN119442)

Target: CD8

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: YTC 182-20

Product Details anti-CD8 Antibody (FITC)

Specificity: This antibody recognises the CD8 cell surface antigen, a 30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.

Immunogen: Mouse L cells transfected with human CD8 gene. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 l whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.027 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

NCBI Accession: NP_001759

UniProt: P01732