CD3 antibody (Cytoplasmic Domain)

Catalog No. ABIN119451

This anti-CD3 antibody is a Rat Monoclonal antibody detecting CD3 in FACS, IHC (fro), IF, WB and IHC (p). Suitable for Human, Mouse, Chicken, Pig, Dog and Horse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for CD3 antibody (Cytoplasmic Domain) (ABIN119451)

Target: CD3

Reactivity: Human, Mouse, Chicken, Pig, Dog, Horse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD3-12

Product Details anti-CD3 Antibody

Binding Specificity: Cytoplasmic Domain

Sequence: Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys

Specificity: This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).

Cross-Reactivity (Details): Species reactivity (expected):Mammals, Birds.
Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic peptide sequence derived from Cytoplasmic epitope of CD3

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the reconstituted antibody undiluted at 2-8 °C.

References for anti-CD3 antibody (ABIN119451)

Hebel, Eisinger, Neumeier, Rein-Fischboeck, Pohl, Meier, Boettcher, Froehner, Adams, Liebisch, Krautbauer, Buechler: "Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." in: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).

Hong, Puaux, Huang, Loumagne, Tow, Mackay, Kato, Prévost-Blondel, Avril, Nardin, Abastado: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." in: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).

Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway