CD163 antibody

Catalog No. ABIN120083

The Mouse Monoclonal anti-CD163 antibody has been validated for WB, FACS, IF, IHC (p), IHC (fro) and EIA. It is suitable to detect CD163 in samples from Human, Monkey and Sheep.

Quick Overview for CD163 antibody (ABIN120083)

Target: CD163

Reactivity: Human, Monkey, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: EDHu-1

Product Details anti-CD163 Antibody

Specificity: This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rhesus Monkey, Sheep.

Purification: Affinity Chromatography on Protein A

Immunogen: Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gene ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7