CD28 antibody (Biotin)

Catalog No. ABIN120437

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS. It is suitable to detect CD28 in samples from Rat.

Quick Overview for CD28 antibody (Biotin) (ABIN120437)

Target: CD28

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: JJ319

Product Details anti-CD28 Antibody (Biotin)

Specificity: Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).

Purification: Protein G Chromatography

Immunogen: Rat CD28 transfected A20J cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 25660

NCBI Accession: NP_037253

UniProt: P31042

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response