CD8 antibody (PE)

Catalog No. ABIN120697

This Mouse Monoclonal antibody specifically detects CD8 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD8 antibody (PE) (ABIN120697)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MEM-31

Product Details anti-CD8 Antibody (PE)

Specificity: The antibody recognizes a conformationally-dependent epitope of CD8 antigen.

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Immunogen: Crude thymus membrane fraction.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry analysis of human blood cells using 20 l reagent / 100 l whole blood or10^6 cells in a suspension. Does not work in Immunohistocchemistry omn paraffin sections or Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Avoid prolonged exposure to light.
Shelf life: one year fromdespatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

UniProt: P01732