TUBA1B antibody

Catalog No. ABIN121117

This Rat Monoclonal antibody specifically detects TUBA1B in WB, IF, IHC (p), IHC (fro), IP, EIA and RIA. It exhibits reactivity toward Human, Mouse, Rat, Avian, Mammalian and Saccharomyces cerevisiae and has been mentioned in 4+ publications.

Quick Overview for TUBA1B antibody (ABIN121117)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human, Mouse, Rat, Avian, Mammalian, Saccharomyces cerevisiae

Host: Rat

Clonality: Monoclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: YOL1-34

Product Details anti-TUBA1B Antibody

Specificity: SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P). This antibody is routinely tested in ELISA on Tubulin.

Purification: Affinity Chromatography on Protein G

Immunogen: Yeast tubulin

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections. ELISA: 10 μg/ul as detecting antibody. RIA. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This antibody is routinely tested in Elisa on Tubulin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-TUBA1B antibody (ABIN121117)

Lang, Grether-Beck, Singh, Kuck, Jakob, Kefalas, Altinoluk-Hambüchen, Graffmann, Schneider, Lindecke, Brenden, Felsner, Ezzahoini, Marini, Weinhold, Vierkötter, Tigges, Schmidt, Stühler, Köhrer et al.: "MicroRNA-15b regulates mitochondrial ROS production and the senescence-associated secretory phenotype through sirtuin 4/SIRT4. ..." in: Aging, Vol. 8, Issue 3, pp. 484-505, (2016) (PubMed).

Takeyama, Yuki, Tokuhara, Oroku, Mejima, Kurokawa, Kuroda, Kodama, Nagai, Ueda, Kiyono: "Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs." in: Vaccine, Vol. 33, Issue 39, pp. 5204-11, (2016) (PubMed).

Kessler, Reich, Jahn, Tolosa, Beck, Kalbacher, Overkleeft, Schempp, Driessen: "Human cytomegalovirus infection interferes with major histocompatibility complex type II maturation and endocytic proteases in dendritic cells at multiple levels." in: The Journal of general virology, Vol. 89, Issue Pt 10, pp. 2427-36, (2008) (PubMed).

Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: alpha Tubulin / TUBA1B

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Pathways: Microtubule Dynamics, M Phase