GAD antibody (C-Term)

Catalog No. ABIN121539

This anti-GAD antibody is a Rabbit Polyclonal antibody detecting GAD in WB, IHC (p), IHC (fro) and EIA. Suitable for Human, Mouse, Rat and Cat.

Quick Overview for GAD antibody (C-Term) (ABIN121539)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human, Mouse, Rat, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-GAD Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Also reacts to: GAD2 / GAD65

Characteristics: Synonyms: Glutamate decarboxylase 1, GAD-67, Glutamate decarboxylase 67 kDa isoform, 67 kDaglutamic acid decarboxylase

Purification: Ammonium Sulphate Precipitation.

Immunogen: Synthetic peptide sequence from the C-terminus of GADAA Sequence: D-F-L-I-E-E-I-E-R-L-G-Q-D-L

Application Details

Application Notes: ELISA. Western Blot: 1/1000-1/10000. Immunohistochemistry on Frozen Sections: 1/200-1/5000. Recommended Positive Control: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1 / GAD67

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

Gene ID: 2571

UniProt: Q99259