BDKRB1 antibody (Cytoplasmic Domain)

Catalog No. ABIN122013

Product Details anti-BDKRB1 Antibody

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDKRB1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reacts with Bradykinin B1 Receptor 1 (family: GPCR, subfamily:Bradykinin)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 5-9 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BDKRB1

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Alternative Name: BDKRB1 (BDKRB1 Products)

Background: Bradykinin Receptor B1 (BKB1) has been implicated in inflammation and nociception. BKB1 receptor is rapidly induced under many inflammatory conditions such as colitis and hyperalgesia, allergic rhinitis, and inflammation. In vitro studies have implicated lipopolysaccharide, interleukins, and interferon as effectors in the rapid induction of BKB1 receptor expression. BKB1 receptor-deficient mice are healthy, fertile, and normotensive but show blunted response to lipopolysaccharide-induced hypotension, reduced migration into inflamed tissues by polymorphonuclear leukocytes, and reduced activity-dependent facilitation of a spinal nociceptive reflex. Bradykinin B1 receptor is generally only minimally expressed under normal physiological conditions, but the expression is induced rapidly under pathologic conditions such as ischemia, atheromatous disease, or exposure to inflammatory cytokines. Expression has been reported in brain, colon, kidney, lung, nasal mucosa, pancreas, prostate, skin, spinal cord, and vessel. ESTs have been isolated from normal human connective tissue libraries and in human kidney and skin cancer libraries.Synonyms: B1 bradykinin receptor, B1R, BK-1 receptor, BKB1R, BKR1, BRADYB1, Bradykinin B1 Receptor

Gene ID: 623

UniProt: P46663

Pathways: ACE Inhibitor Pathway