Mrgprf antibody (Cytoplasmic Domain)

Catalog No. ABIN122237

The Rabbit Polyclonal anti-Mrgprf antibody has been validated for IHC (p). It is suitable to detect Mrgprf in samples from Human.

Quick Overview for Mrgprf antibody (Cytoplasmic Domain) (ABIN122237)

Target: Mrgprf (MAS-Related GPR, Member F (Mrgprf))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mrgprf antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Mrgprf Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Reacts with MAS-related GPR, member F (MRGPRF) (family: GPCR, subfamily: Orphan-U)

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 13 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Mrgprf

Target: Mrgprf (MAS-Related GPR, Member F (Mrgprf))

Alternative Name: GPR140

Background: MAS-related GPR, member F (MRGPRF) is an Orphan-U GPCR with an unknown ligand. MAS-related GPR, member F (MRGPRF) has been reported in rat gut, vas deferens, uterus, and aorta, but not in liver, kidney, lung, or salivary gland. Human ESTs have been isolated from brain, breast, colon, connective tissue, heart, liver/spleen, lung, placenta, prostate, spleen, testis, and uterus libraries.Synonyms: G-protein coupled receptor 140, GPR168, MRGF, MRGPRF, Mas-related G-protein coupled receptor member F, Mas-related gene F protein, PSEC0142

Gene ID: 116535

UniProt: Q96AM1