Psaf antibody

Catalog No. ABIN125858

The Rabbit Polyclonal anti-Psaf antibody has been validated for WB. It is suitable to detect Psaf in samples from Arabidopsis thaliana, Barley, Nicotiana tabacum, Oryza sativa and Spinach. There are 2+ publications available.

Quick Overview for Psaf antibody (ABIN125858)

Target: Psaf (psi-F Subunit of Photosystem I (Psaf))

Reactivity: Arabidopsis thaliana, Barley, Nicotiana tabacum, Oryza sativa, Spinach

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Psaf Antibody

Cross-Reactivity: Barley (Hordeum vulgare)

Cross-Reactivity (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus PCC 7942

Characteristics: Expected / apparent Molecular Weight of the Antigene: 24 kDa / 17 kDa for Arabidopsis thaliana

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Psaf antibody (ABIN125858)

Sakuraba, Rahman, Cho, Kim, Koh, Yoo, Paek: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).

Krech, Ruf, Masduki, Thiele, Bednarczyk, Albus, Tiller, Hasse, Schöttler, Bock: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).

Target Details for Psaf

Target: Psaf (psi-F Subunit of Photosystem I (Psaf))

Background: AGI Code: At1g31330
PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

Molecular Weight: expected: 24 kDa , apparent: 17 kDa for Arabidopsis thaliana

UniProt: Q9SHE8