Psaf antibody

Catalog No. ABIN125858

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Psaf antibody is suitable to detect Psaf in samples from Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum, Oryza sativa and Spinach. It has been validated for WB.

Quick Overview for Psaf antibody (ABIN125858)

Target: Psaf (psi-F Subunit of Photosystem I (Psaf))

Reactivity: Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Nicotiana tabacum, Oryza sativa, Spinach

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Psaf Antibody

Purpose: Anti-PsaF | PSI-F subunit of photosystem I (plant)

Cross-Reactivity: Barley (Hordeum vulgare)

Cross-Reactivity (Details): Arabidopsis thaliana, Briopsis corticulans, Hordeum vulgare (very weak), Nicotiana tabaccum, Oryza sativa, Spinacia oleracea
Not reactive in: Chlamydomonas reinhardtii, Synechococcus PCC 7942

Predicted Reactivity: Catalpa bungei,Micromonas sp.,Populus trichocarpa,Physcomitrium patens,Ricinus communis

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Psaf antibody (ABIN125858)

Sakuraba, Rahman, Cho, Kim, Koh, Yoo, Paek: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).

Krech, Ruf, Masduki, Thiele, Bednarczyk, Albus, Tiller, Hasse, Schöttler, Bock: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).

Target Details for Psaf

Target: Psaf (psi-F Subunit of Photosystem I (Psaf))

Background: PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

Molecular Weight: 24 kDa | 17 kDa for Arabidopsis thaliana

Gene ID: 840021

UniProt: Q9SHE8