DNA
ELISA, IF, DB
Host: Mouse
Monoclonal
163-47
unconjugated
Recombinant Antibody
Application Notes
Immunohistochemistry on frozen and paraffin embedded tissue: 1: 10(after reconstitution dilute further with PBS), incubation time 1h at RT. Dot blot on nitrocellulose membrane (after baking at 70C). Cell culture. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 1 mL distilled water
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
DsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.Synonyms: Deoxyribonucleic Acid