LYVE1 antibody (AA 24-228) (Biotin)

Catalog No. ABIN126137

The Rabbit Polyclonal anti-LYVE1 antibody has been validated for WB, FACS, IHC (p), EIA and IHC (fro). It is suitable to detect LYVE1 in samples from Mouse.

Quick Overview for LYVE1 antibody (AA 24-228) (Biotin) (ABIN126137)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYVE1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LYVE1 Antibody (Biotin)

Binding Specificity: AA 24-228

Specificity: Highly specifc for Mouse Lyve-1.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Antigen Affinity Chromatography

Immunogen: Highly pure recombinant Mouse soluble LYVE-1 produced in insect cells (Cat.-No DA3524). Remarks: The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).

Application Details

Application Notes: The unconjugated antibody can be used for ELISA (1-15 μg/mL), Western blotting (1-2 μg/mLwith the appropriate secondary reagents), FACS (3-20 μg/mL together with the appropriatesecondary reagents) and Cell sorting. The antibody is also suitable for Immunohistochemistry on on Paraffin as well as on FrozenSections (0.25-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with sterile water to a concentration of 0.1-1.0 μg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.4, 0.02 % Sodium Azide, 50x BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: LYVE-1

Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gene ID: 114332

NCBI Accession: NP_444477

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process