c-FOS antibody (N-Term)

Catalog No. ABIN126801

This anti-c-FOS antibody is a Mouse Monoclonal antibody detecting c-FOS in WB, IHC (fro) and EIA. Suitable for Human, Rat, Mouse and Dog.

Quick Overview for c-FOS antibody (N-Term) (ABIN126801)

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 8B5

Product Details anti-c-FOS Antibody

Binding Specificity: N-Term

Specificity: This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.

Characteristics: Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch

Expiry Date: 12 months

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: C-Fos

Background: The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

Gene ID: 2353

UniProt: P01100

Pathways: S100 Proteins