Phosphoserine antibody

Catalog No. ABIN126866

The Mouse Polyclonal anti-Phosphoserine antibody has been validated for WB, IP and EIA. It is suitable to detect Phosphoserine in samples from .

Quick Overview for Phosphoserine antibody (ABIN126866)

Target: Phosphoserine

Reactivity: Please inquire

Host: Mouse

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Phosphoserine Antibody

Purification: Purified

Components: incl. positive Control

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: Positive Control: pSer / pThr Molecular Weight MarkerFormulation: The pSer/pThr molecular weight marker contains rabbit muscle phosphoproteins isolatedby Fe3+/IDA - affinity chromatography. Proteins are lyophilized fromPBS/NaF/PEG/Sucrose/ Bromophenolblue and Na - azide. After reconstitution the solutioncontains 0. 09% Na-azide. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The pSer/pThr molecular weight marker is recommended for immunoblot applications. Use20µl molecular weight marker per lane. Note: Use BSA based blot incubation buffers. Milk,Casein and Blotto might interfere with antibody - antigen interaction.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphoserine

Target: Phosphoserine

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains. Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.