DDIT3 antibody

Catalog No. ABIN127103

The Rabbit Polyclonal anti-DDIT3 antibody has been validated for WB, IP and IHC (p). It is suitable to detect DDIT3 in samples from Human, Mouse and Rat.

Quick Overview for DDIT3 antibody (ABIN127103)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDIT3 Antibody

Specificity: The antibody recognizes 25 kDa GADD135.

Purification: Protein A affinity chromatography

Immunogen: Synthetic peptide surrounding amino acid 124 of human GADD153

Application Details

Application Notes: Western blotting (0.5-4 μg/mL). Immunoprecipitation (10-20 μg/mL). Immunohistochemistry (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated freeze/defrost cycles.

Storage: -20 °C

Storage Comment: Store at -20 °C for up to one year from despatch. For longer storage, freeze at -70 °C.

Expiry Date: 12 months

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: GADD153 / CHOP

Background: GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from Synonyms: C/EBP-homologous protein, C/EBP-homologous protein 10, CHOP-10, CHOP10, DDIT-3, DDIT3, DNA damage-inducible transcript 3, Growth arrest and DNA-damage-inducible protein GADD153

Gene ID: 1649

NCBI Accession: NP_001181986

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis