MMP13 antibody

Catalog No. ABIN127179

This anti-MMP13 antibody is a Rabbit Polyclonal antibody detecting MMP13 in WB, IP and IHC (p). Suitable for Human, Mouse, Rat, Rabbit, Cow and Horse.

Quick Overview for MMP13 antibody (ABIN127179)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MMP13 Antibody

Specificity: The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Equine (Horse), Rabbit and Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 454 of Rat MMP-13.

Application Details

Application Notes: Western blotting (0.5-4 μg/mL) . Immunoprecipitation. Immunohistochemistry (works with Paraffin Embedded Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13

Background: The Matrix Metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen type I, II, III.Synonyms: Collagenase 3, MMP13, Matrix metalloproteinase-13

Gene ID: 4322

NCBI Accession: NP_002418

UniProt: P45452