HDAC1 antibody (C-Term)

Catalog No. ABIN127232

The Rabbit Polyclonal anti-HDAC1 antibody has been validated for WB, IP and IHC (p). It is suitable to detect HDAC1 in samples from Human, Mouse and Rat.

Quick Overview for HDAC1 antibody (C-Term) (ABIN127232)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC1 Antibody

Binding Specificity: C-Term

Specificity: The antibody detects the 62 kDa histone deacetylase 1. It does not cross-react with other HDAC proteins including HDAC2, 3, 4, 5, 6, 7, and 8.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to the C-terminus of human HDAC1

Application Details

Application Notes: Western blotting (0.5-4 μg/mL). Immunoprecipitation (10-20 μg/mL). Immunohistochemistry (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated freeze/defrost cycles.

Storage: -20 °C

Storage Comment: Store at -20 °C. For long-term storage, aliquot and freeze at -70 °C.

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: HDAC1 is an active component of transcriptional corepressor complexes which can be recruited to specific promoter regions via transcriptional factors. HDAC1 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, which leads to the inhibition of gene transcription.Synonyms: HD1, Histone deacetylase 1, RPD3L1

Gene ID: 3065

NCBI Accession: NP_004955

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis