ANKRD32 antibody (AA 75-100)

Catalog No. ABIN129614

Supplier Product No.: 600-401-681

Product Details anti-ANKRD32 Antibody

Target: ANKRD32 (Ankyrin Repeat Domain 32 (ANKRD32))

Binding Specificity: AA 75-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANKRD32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-681

Supplier: Rockland

Purpose: HALLEN Antibody

Characteristics: Synonyms: rabbit anti-HALLEN antibody, Ankyrin repeat domain-containing protein 32, ANKRD32, cDNA FLJ57532, BRCT domain-containing protein 1, BRCTD1 antibody, Smc5/6 localization factor 1, SLF1, SMC5-SMC6 Complex Localization Factor 1

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 75-100 of Human Hallen.  Hallen is a novel BRCT domain containing protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.33 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ANKRD32

Target: ANKRD32 (Ankyrin Repeat Domain 32 (ANKRD32))

Alternative Name: SLF1 (ANKRD32 Products)

Background: Background: Anti-HALLEN plays a role in the DNA damage response pathway by regulating post replication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links and DNA double-strand breaks sites on chromatin during DNA repair in response to stalled replication forks. Anti-HALLEN promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions. Anti-Hallen Antibody is useful for researchers interested in DNA damage and repair.

Gene ID: 84250

NCBI Accession: NP_115666

UniProt: Q9BQI6