HIS2AV (pSer137) antibody

Catalog No. ABIN129671

Supplier Product No.: 600-401-914

This Rabbit Polyclonal antibody specifically detects in ELISA, IHC, WB. It exhibits reactivity toward Drosophila melanogaster and has been mentioned in 27+ publications.

Quick Overview for HIS2AV (pSer137) antibody (ABIN129671)

Target: HIS2AV

Reactivity: Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: pSer137

Supplier Product No.: 600-401-914

Supplier: Rockland

Purpose: Histone H2AvD phosphoS137 Antibody

Cross-Reactivity (Details): Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue.

Characteristics: Synonyms: rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 μg/mL

Application Note: Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract.  Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS137 of H2AvD protein.

Western Blot Dilution: 1:400 - 1:1,600

ELISA Dilution: 1:29,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Lin, Wang, Lin, Rastegari, Su, Chang, Liao, Chang, Pi, Yu, Chen, Lin, Lu, Su, Tzou, Chan, Hsu: "Piwi reduction in the aged niche eliminates germline stem cells via Toll-GSK3 signaling." in: Nature communications, Vol. 11, Issue 1, pp. 3147, (2020) (PubMed).

Perlmutter, Bordenstein, Unckless, LePage, Metcalf, Hill, Martinez, Jiggins, Bordenstein: "The phage gene wmk is a candidate for male killing by a bacterial endosymbiont." in: PLoS pathogens, Vol. 15, Issue 9, pp. e1007936, (2020) (PubMed).

Grendler, Lowgren, Mills, Losick: "Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage." in: Development (Cambridge, England), Vol. 146, Issue 15, (2020) (PubMed).

Tsakiri, Gumeni, Vougas, Pendin, Papassideri, Daga, Gorgoulis, Juhász, Scorrano, Trougakos: "Proteasome dysfunction induces excessive proteome instability and loss of mitostasis that can be mitigated by enhancing mitochondrial fusion or autophagy." in: Autophagy, Vol. 15, Issue 10, pp. 1757-1773, (2020) (PubMed).

Mlih, Khericha, Birdwell, West, Karpac: "A virus-acquired host cytokine controls systemic aging by antagonizing apoptosis." in: PLoS biology, Vol. 16, Issue 7, pp. e2005796, (2019) (PubMed).

Cosolo, Jaiswal, Csordás, Grass, Uhlirova, Classen: "JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress." in: eLife, Vol. 8, (2019) (PubMed).

Merigliano, Mascolo, La Torre, Saggio, Vernì: "Protective role of vitamin B6 (PLP) against DNA damage in Drosophila models of type 2 diabetes." in: Scientific reports, Vol. 8, Issue 1, pp. 11432, (2018) (PubMed).

Harumoto, Lemaitre: "Male-killing toxin in a bacterial symbiont of Drosophila." in: Nature, Vol. 557, Issue 7704, pp. 252-255, (2018) (PubMed).

McCarthy, Deiulio, Martin, Upadhyay, Rangan: "Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila." in: Molecular biology of the cell, Vol. 29, Issue 24, pp. 2933-2945, (2018) (PubMed).

Caridi, DAgostino, Ryu, Zapotoczny, Delabaere, Li, Khodaverdian, Amaral, Lin, Rau, Chiolo: "Nuclear F-actin and myosins drive relocalization of heterochromatic breaks." in: Nature, Vol. 559, Issue 7712, pp. 54-60, (2018) (PubMed).

Koehler, Perkins, Ellisman, Jones: "Pink1 and Parkin regulate Drosophila intestinal stem cell proliferation during stress and aging." in: The Journal of cell biology, Vol. 216, Issue 8, pp. 2315-2327, (2017) (PubMed).

Merigliano, Marzio, Renda, Somma, Gatti, Vernì: "A Role for the Twins Protein Phosphatase (PP2A-B55) in the Maintenance of Drosophila Genome Integrity." in: Genetics, Vol. 205, Issue 3, pp. 1151-1167, (2017) (PubMed).

Ma, Han, Song, Do, Yang, Ni, Xie: "DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation." in: Development (Cambridge, England), Vol. 143, Issue 23, pp. 4312-4323, (2017) (PubMed).

Chen, Zheng, Xiao, Zheng: "Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence." in: Aging cell, Vol. 15, Issue 3, pp. 542-52, (2017) (PubMed).

Swenson, Colmenares, Strom, Costes, Karpen: "The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic." in: eLife, Vol. 5, (2017) (PubMed).

Delabaere, Ertl, Massey, Hofley, Sohail, Bienenstock, Sebastian, Chiolo, LaRocque: "Aging impairs double-strand break repair by homologous recombination in Drosophila germ cells." in: Aging cell, Vol. 16, Issue 2, pp. 320-328, (2017) (PubMed).

Bayona-Feliu, Casas-Lamesa, Reina, Bernués, Azorín: "Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin." in: Nature communications, Vol. 8, Issue 1, pp. 283, (2017) (PubMed).

Hong, Choi: "Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences." in: Nature communications, Vol. 7, pp. 12988, (2016) (PubMed).

Upadhyay, Martino Cortez, Wong-Deyrup, Tavares, Schowalter, Flora, Hill, Nasrallah, Chittur, Rangan: "Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila." in: PLoS genetics, Vol. 12, Issue 3, pp. e1005918, (2016) (PubMed).

Ryu, Spatola, Delabaere, Bowlin, Hopp, Kunitake, Karpen, Chiolo: "Heterochromatic breaks move to the nuclear periphery to continue recombinational repair." in: Nature cell biology, Vol. 17, Issue 11, pp. 1401-11, (2016) (PubMed).

Target Details

Target: HIS2AV

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known.  H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD  is expressed both maternally and zygotically and is found in embryos through to adults (female only).  The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur.  In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.

Gene ID: 43229, 17738227

UniProt: P08985