Methylated Lysine antibody

Catalog No. ABIN129693

Supplier Product No.: 600-401-940

Product Details

Target: Methylated Lysine

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-940

Supplier: Rockland

Purpose: Lysine Methylated Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Characteristics: Synonyms: Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays.  Although not tested, this antibody is likely functional in RIA, and flow cytometry.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Methylated Lysine

Alternative Name: Lysine Methylated

Target Type: Chemical

Background: Background: Anti-Lysine Methylated (MeK) Antibody is used in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.