ABCB6 antibody (AA 430-465)

Catalog No. ABIN129698

Supplier Product No.: 600-401-945

The Rabbit Polyclonal anti-ABCB6 antibody has been validated for WB and ELISA. It is suitable to detect ABCB6 in samples from Human and Dog. There are 2+ publications available.

Quick Overview for ABCB6 antibody (AA 430-465) (ABIN129698)

Target: ABCB6 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ABCB6 Antibody

Binding Specificity: AA 430-465

Supplier Product No.: 600-401-945

Supplier: Rockland

Purpose: ABCB6 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human ABCB6 protein.

Characteristics: Synonyms: rabbit anti-ABCB6 Antibody, ABCB 6, ABCB-6, ABC transporter umat antibody, ABC14 antibody, ATP binding cassette sub family B member 6, mitochondrial precursor antibody, mitochondrial precursor ABC transporter 3, Mt-ABC transporter 3

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 430-465 of human ABCB6 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94 kDa in size corresponding to ABCB6 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:40,000 - 1:130,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ABCB6 antibody (ABIN129698)

Nakayama, Otsuka, Kobayashi, Okajima, Matsumoto, Hagiya, Inoue, Shuin, Nakajima, Tanaka, Ogura: "Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy." in: Scientific reports, Vol. 6, pp. 36478, (2016) (PubMed).

Fukuda, Aguilar-Bryan, Vaxillaire, Dechaume, Wang, Dean, Moitra, Bryan, Schuetz: "Conserved intramolecular disulfide bond is critical to trafficking and fate of ATP-binding cassette (ABC) transporters ABCB6 and sulfonylurea receptor 1 (SUR1)/ABCC8." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 8481-92, (2011) (PubMed).

Target Details for ABCB6

Target: ABCB6 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6))

Alternative Name: ABCB6

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ABCB6 (ATP-binding cassette sub-family B member 6) is a member of the superfamily of ATP-binding cassette (ABC) transporters.  ABCB6 is a membrane-associated protein that transports various molecules across the extra- and intra-cellular membranes.  ABC genes are divided into seven distinct subfamilies (ABC1, DR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily confer multi-drug resistance (MDR) to cancer cells and are also involved in antigen presentation. ABCB6 also likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function. Anti-ABCB6 is ideal for researchers interested in heme synthesis.

Gene ID: 10058, 13123949

UniProt: Q9NP58

Pathways: Transition Metal Ion Homeostasis