CNTNAP1 antibody (Cytoplasmic Domain)

Catalog No. ABIN1304571

This anti-CNTNAP1 antibody is a Mouse Monoclonal antibody detecting CNTNAP1 in WB, IHC, IP and ICC. Suitable for Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for CNTNAP1 antibody (Cytoplasmic Domain) (ABIN1304571)

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: K65-35

Product Details anti-CNTNAP1 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: No off-targets reported

Cross-Reactivity: Gerbil, Human, Mouse, Rat

Characteristics:

Description: Our Anti-CASPR/Neurexin IV mouse monoclonal primary antibody is produced in-house from hybridoma clone K65/35. It is KO validated, detects gerbil, human, mouse, and rat CASPR/Neurexin IV, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our CASPR/Neurexin IV mouse monoclonal primary antibody from hybridoma clone K65/35. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein 1308-1381 (cytoplasmic domain) of rat Caspr (accession number P97846) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: IHC: 1:250

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

References for anti-CNTNAP1 antibody (ABIN1304571)

McGonigal, Barrie, Yao, Black, McLaughlin, Willison: "Neuronally expressed a-series gangliosides are sufficient to prevent the lethal age-dependent phenotype in GM3-only expressing mice." in: Journal of neurochemistry, (2021) (PubMed).

Target Details for CNTNAP1

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Alternative Name: Neurexin IV

Background:

Synonyms: Contactin-associated protein 1 (Caspr) (Caspr1) (Neurexin IV) (Neurexin-4) (Paranodin) (p190)

Target Description: CASPR (Neurexin IV) is part of the neurexin family, which includes presynaptic proteins located in the presynaptic membrane of the brain.

Gene Name Alternatives: Cntnap1 Caspr Nrxn4

Molecular Weight: 220 kDa

UniProt: P97846

Pathways: Cell-Cell Junction Organization