GIT1 antibody (AA 375-770)

Catalog No. ABIN1304673

This anti-GIT1 antibody is a Mouse Monoclonal antibody detecting GIT1 in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for GIT1 antibody (AA 375-770) (ABIN1304673)

Target: GIT1

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: N39B-8

Product Details anti-GIT1 Antibody

Binding Specificity: AA 375-770

Specificity: No off-targets reported for GIT2

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-GIT1 mouse monoclonal primary antibody is produced in-house from hybridoma clone N39B/8. It is KO validated, detects human, mouse, and rat GIT1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our GIT1 mouse monoclonal primary antibody from hybridoma clone N39B/8. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(accession number Q9Z272) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background:

Synonyms: ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1)

Target Description: G-protein coupled receptor kinase interacting ArfGAP 1 is encoded by the gene GIT1. GIT1 is a member of the GIT protein family. GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. GIT1 is expressed in numerous tissues with the highest levels in the testis. Diseases associated with GIT1 include Attention Deficit-Hyperactivity Disorder.

Gene Name Alternatives: Git1

Molecular Weight: 90 kDa

UniProt: Q9Z272