HCN4 antibody (AA 1019-1108)

Catalog No. ABIN1304702

The Mouse Monoclonal anti-HCN4 antibody has been validated for WB, IHC and ICC. It is suitable to detect HCN4 in samples from Rat.

Quick Overview for HCN4 antibody (AA 1019-1108) (ABIN1304702)

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N114-10

Product Details anti-HCN4 Antibody

Binding Specificity: AA 1019-1108

Specificity: No off-targets reported for other HCN's

Cross-Reactivity: Gerbil, Human, Mouse, Pig, Rat

Characteristics:

Description: Our Anti-HCN4 mouse monoclonal primary antibody is produced in-house from hybridoma clone N114/10. It detects human, mouse, and rat HCN4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Manufacturer Comment: We produce our HCN4 mouse monoclonal primary antibody from hybridoma clone N114/10. It is great in IHC, ICC, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession number Q9JKA7) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: IHC: 1:200

Dilution Range: ICC: 1:200

Dilution Range: WB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4

Background:

Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Target Description: HCN4 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN4 is found in the cell membrane as a multi pass membrane protein in the sino-atrial node of the heart. This protein is highly expressed in neurons in the thalamus and other brain regions and testis. Mutations in HCN4 have been found in several types of sinus bradycardia.

Gene Name Alternatives: HCN4

Molecular Weight: 130 kDa

UniProt: Q9JKA7