SHANK2 antibody (AA 84-309)

Catalog No. ABIN1304903

The Mouse Monoclonal anti-SHANK2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SHANK2 in samples from Rat.

Quick Overview for SHANK2 antibody (AA 84-309) (ABIN1304903)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: N23B-49

Product Details anti-SHANK2 Antibody

Binding Specificity: AA 84-309

Specificity: Recognizes Shank1, Shank2 and Shank3

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-Pan-Shank mouse monoclonal primary antibody is produced in-house from hybridoma clone N23B/49. It detects human, mouse, and rat Pan-Shank, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our Pan-Shank mouse monoclonal primary antibody from hybridoma clone N23B/49. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (accession number Q9QX74) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: IHC: 1:500

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: Shank

Background:

Synonyms: SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon) SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3) SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Target Description: This antibody is a pan antibody that recognizes SH3 and multiple ankyrin repeat domains protein 1 (Shank 1), SH3 and multiple ankyrin repeat domains protein 2 (Shank 2) and SH3 and multiple ankyrin repeat domains protein 3 (Shank3). Shank proteins act as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling.

Gene Name Alternatives: Shank1 Shank2 Cortbp1 Kiaa1022 PROSAP1 Shank3 Kiaa1650 Prosap2 PSAP2

Molecular Weight: 160 kDa