L-Selectin antibody

Catalog No. ABIN135253

This Rat Monoclonal antibody specifically detects L-Selectin in FACS. It exhibits reactivity toward Mouse.

Quick Overview for L-Selectin antibody (ABIN135253)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: MEL-14

Product Details anti-L-Selectin Antibody

Specificity: Mouse CD62L, Mr 74 kDa on lymphocytes and Mr 95 kDa on neutrophils

Characteristics: Rat Anti-Mouse CD62L-LE/AF

Purification: boric acid precipitation

Endotoxin Level: Low endotoxin

Immunogen: C3H/eb cloned mouse B cell lymphoma 38C-13

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PACBLU conjugate 0.3 g/106 cells PE, APC, SPRD, PE/CY7, APC/CY7, AF488, AF647, and AF700 0.1 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vivo and in vitro blocking of adhesion, Cytotoxicity studies

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L

Target Type: Chemical

Background: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.