CD86 antibody (PE)

Catalog No. ABIN135276

This anti-CD86 antibody is a Rat Monoclonal antibody detecting CD86 in FACS. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD86 antibody (PE) (ABIN135276)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: GL1

Product Details anti-CD86 Antibody (PE)

Specificity: Mouse CD86, Mr 80 kDa

Characteristics: Rat Anti-Mouse CD86-PE

Immunogen: LPS-activated CBA/Ca mouse spleen B cells

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vivo and in vitro blocking assays

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD86 antibody (PE) (ABIN135276)

Andersen, Huszthy, Gopalakrishnan, Jacobsen, Fauskanger, Tveita, Grødeland, Bogen: "Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules." in: NPJ vaccines, Vol. 4, pp. 9, (2019) (PubMed).

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation