H-2Kd/H-2Dd antibody

Catalog No. ABIN135416

The Mouse Monoclonal anti- antibody has been validated for FACS. It is suitable to detect in samples from Mouse.

Quick Overview for H-2Kd/H-2Dd antibody (ABIN135416)

Target: H-2Kd/H-2Dd

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Clone: 34-1-2S

Product Details

Specificity: Mouse H-2Kd/H-2Dd

Characteristics: Mouse Anti-Mouse H-2Kd/H-2Dd-UNLB

Purification: Purified

Immunogen: BDF1 mouse splenocytes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , ICC - Reported in literature , EM - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Blocking Cell Adhesion, Complement-mediated cytotoxicity

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: H-2Kd/H-2Dd

Background: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.