CD3 epsilon antibody (PE)

Catalog No. ABIN135467

The Mouse Monoclonal anti-CD3 epsilon antibody has been validated for FACS. It is suitable to detect CD3 epsilon in samples from Pig. There are 2+ publications available.

Quick Overview for CD3 epsilon antibody (PE) (ABIN135467)

Target: CD3 epsilon (CD3E)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: PPT3

Product Details anti-CD3 epsilon Antibody (PE)

Specificity: Porcine CD3, Mr 23 kDa

Characteristics: Mouse Anti-Porcine CD3e-PE

Immunogen: Purified CD3 molecules from porcine thymus

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature , Stim - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 epsilon antibody (PE) (ABIN135467)

Chen, Hou, Li, Xiong, Liu: "Cloning and characterization of porcine 4Ig-B7-H3: a potent inhibitor of porcine T-cell activation." in: PLoS ONE, Vol. 6, Issue 6, pp. e21341, (2011) (PubMed).

Hosoya, Villena, Shimazu, Tohno, Fujie, Chiba, Shimosato, Aso, Suda, Kawai, Saito, Alvarez, Ikegami, Itoh, Kitazawa: "Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells." in: Veterinary research, Vol. 42, pp. 111, (2011) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway