CD8 alpha antibody

Catalog No. ABIN135476

The Mouse Monoclonal anti-CD8 alpha antibody has been validated for FACS. It is suitable to detect CD8 alpha in samples from Pig.

Quick Overview for CD8 alpha antibody (ABIN135476)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 76-2-11

Product Details anti-CD8 alpha Antibody

Specificity: Porcine CD8α, Mr 35 kDa

Characteristics: Mouse Anti-Porcine CD8a-UNLB

Purification: Purified

Immunogen: Fresh dd miniature swine thymocytes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Cytotoxicity assays, In vivo depletion of CD8+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or a CD8 heterodimer. It is found on most thymocytes, and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.

Pathways: TCR Signaling