TCRgd antibody (Biotin)

Catalog No. ABIN135518

The Mouse Monoclonal anti-not_set antibody has been validated for FACS. It is suitable to detect not_set in samples from Chicken. There are 2+ publications available.

Quick Overview for TCRgd antibody (Biotin) (ABIN135518)

Target: TCRgd

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: TCR-1

Product Details

Specificity: Chicken/Milk Snake TCR, Mr 50 & 40 kDa

Characteristics: Mouse Anti-Chicken TCRgd-BIOT

Immunogen: Outbred chicken thymocytes and Ig-negative blood lymphocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In ovo depletion of gd T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Han, Renu, Patil, Schrock, Feliciano-Ruiz, Selvaraj, Renukaradhya: "Immune Response to Salmonella Enteritidis Infection in Broilers Immunized Orally With Chitosan-Based Salmonella Subunit Nanoparticle Vaccine." in: Frontiers in immunology, Vol. 11, pp. 935, (2020) (PubMed).

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

Target Details

Target: TCRgd